![]() ![]() Was extracted by the Qiagen 9600 liquid handling robot and the G/mL) -25% glycerol mixture and 75 L of cells. Prepared from the cell cultures with 75 L of a LB-amp (100 We grew pBluescript SK (+) phagemids overnight at 37C andĢ40 rpm in 96-well culture plates containing 1.7 mL of LB broth, Recovered 5,760 bacterial clones containing excised pBluescript SK (+) Subsequently plated on LB agar containing 100 g/mL ampicillin. An aliquot of the excised,Īmplified library was used for infecting XL1-Blue MRF cells and Mass excision of the amplified library was carried out byĮx-Assist helper phage (Stratagene). Library was generated with a titer of 1.0 x 109 plaqueforming units per Gigapack III Gold Packaging Extract (Stratagene). The resulting DNA was packaged into Lambda particles with Vector with Stratagene's ZAP-cDNA Synthesis Kit (Stratagene, La Poly (A) + RNA was purified with the Poly (A) Pure kitĪccording to the manufacturer's instructions (Ambion, Austin, TX).Ī directional cDNA library was constructed in Lambda Uni-ZAP XR Salt-phenol-chloroform procedure as previously described by Strommer etĪl. In liquid nitrogen and total RNA was extracted with guanidinium Trees in Picos Farm in Fort Pierce, FL in 2005 to investigate theīiology and pathology of adult psyllids. citri.Ī cDNA library was constructed from wild adult D. This is the first report of a virus in D. Had significant homology to viruses within the family Reoviridae, genus, In this study, annotation of sequences extracted from whole D.Ĭitri resulted in the discovery and validation of viral sequence, which ![]() Shown to provide environmentally friendly management tools for insect (Lymantria dispar), and the Douglas fir tussock moth (Orgria Suppress the corn earworm moth (Heliothis zea), the Asian gypsy moth In the United States, Nuclear polyhedrosis viruses (NPVs) are used as insecticides (Thorne et al. Viruses, and many viruses have been applied as insecticides (Tweeten etĪl. Like most animals, insects are susceptible to diseases caused by However, widespread use ofĬhemical insecticides will result in the eventual development of Currently chemicalĬontrol is the easiest method of control. Integrated pest management approaches (Hoy 1998). citri populations will require multi-tactic With a severe disease in citrus trees, Huanglongbing, HLB (also known asĬitrus Greening disease), and threatens the viability and production ofĬitrus worldwide (Halbert & Manjunath 2004). Is the most serious pest of citrus, due to the ability to transmitĬandidatus Liberibacter asiaticus, CLa, a bacterium found in association The Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Psyllidae) APA style: Reovirus-like sequences isolated from adult Asian citrus psyllid, (Hemiptera: Psyllidae: Diaphorina citri).Reovirus-like sequences isolated from adult Asian citrus psyllid, (Hemiptera: Psyllidae: Diaphorina citri)." Retrieved from 2009 Florida Entomological Society 15 Oct. MLA style: "Reovirus-like sequences isolated from adult Asian citrus psyllid, (Hemiptera: Psyllidae: Diaphorina citri)." The Free Library.
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